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演讲摘要：Phase separation is an important mechanism that mediates the spatial distribution of proteins in different cellular compartments. While phase-separated proteins share certain sequence characteristics, including intrinsically disordered regions (IDRs) and prion-like domains (PLDs), such characteristics are insufficient for making accurate predictions; thus, a proteome-wide understanding of phase separation is currently lacking. Here, we define phase-separated proteomes based on the systematic analysis of immunofluorescence (IF) images of 12073 proteins in the Human Protein Atlas. The analysis of these proteins reveals that phase-separated candidate proteins exhibit higher IDR contents, higher mean net charge, lower hydropathy, and prefer to bind to RNA. Kinases and transcription factors are also enriched among these candidate proteins. Strikingly, both phase-separated kinases and phase-separated transcription factors display significantly reduced substrate specificity. Our work provides the first global view of the phase-separated proteome and suggests that the spatial proximity resulting from phase separation reduces the requirement for motif specificity and expands the repertoire of substrates.
讲者简介：北京大学基础医学院副教授、博士生导师，清华大学生物信息学博士，主要研究方向为蛋白质翻译后修饰网络及生物大分子凝聚体。在Nucleic Acids Research、Cell Research、Nature commutations等期刊发表SCI论文43篇，SCI他引次数共计1091次，H因子20。作为课题负责人承担国家重点研发计划蛋白质专项，作为负责人承担国家自然科学基金4项，作为骨干参加973计划等国家级课题2项。担任中国人工智能学会生物信息学与人工生命专委会委员、中国计算机学会生物信息学专委会委员、中国生物工程学会青年工作委员会委员、第一届生物物理学会生物大分子相分离与相变分会理事。
第五届中国计算机学会生物信息学会议
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